Molecular detection of aminoglycosides modifying enzymes and its relationship to drug resistance in Pseudomonas aeruginosa

Al-janaby, Sara Hameed; Shehan, Mayada Abdullah

doi:10.37652/juaps.2023.141186.1088

Document Type : Research Paper

Authors

¹ Master's student / Anbar University

² Department of Biology, College of Science, University of Anbar, Anbar,Iraq;

https://doi.org/10.37652/juaps.2023.141186.1088

Abstract

Pseudomonas aeruginosa is an opportunistic pathogen that causes a variety of infections throughout the world, including bacteremia in immunocompromised patients, wound and soft tissue infections, hospital-acquired pneumonia, respiratory infections, and urinary tract infections. It is also the primary cause of life-threatening infections in burn patients. A total of 120 specimens were collected from Al-Anbar Province hospitals, they were obtained from a wound (65.85%), burns (52.63), UTI (33.33%),and sputum (48.27%). Out of these specimens, 65 isolates were bacteriologically identified as P.aeruginosa depending on cultural and microscopical properties, automated (VITEK-2 system), and molecular identification based on 16SrRNA gene, which is an essential gene expressed isolates in all P.aeruginosa. The antimicrobial susceptibility test was done by using the disc diffusion method of aminoglycoside antimicrobials. The results appeared the highest resistance to Gentamycin (81.53%), Amikacin (73.84%), then Tobramycin (66.15%), and Netilmicin (33.84%). Molecular analysis of aminoglycosides modifying enzymes genes showed that the percentage of the AAC(6 ′ )-Ib gene was highest 16/20 (85%), then 8/20 (40%), 2/20 (10%) for ANT (3 ″ )-Ia , APH(3 ′ )-IIb, respectively.

Keywords

Main Subjects

Biology

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Journal of University of Anbar for Pure Science

Molecular detection of aminoglycosides modifying enzymes and its relationship to drug resistance in Pseudomonas aeruginosa

References

References

Volume 17, Issue 2 - Issue Serial Number 2
December 2023
Page 5-11

Molecular detection of aminoglycosides modifying enzymes and its relationship to drug resistance in Pseudomonas aeruginosa

References

References

Volume 17, Issue 2 - Issue Serial Number 2December 2023Page 5-11

Volume 17, Issue 2 - Issue Serial Number 2
December 2023
Page 5-11