Document Type : Research Paper
Authors
Department of Chemistry, College of Science, Al-Nahrain University, Baghdad, Iraq
Abstract
The objective of this investigation was to study the effect of artificial ultraviolet (UV) radiation of varying wavelengths on the degradation of whole human blood DNA and semen over varying time lengths of exposure. DNA degrades rapidly when exposed to environmental factors like high temperature and UV-radiation. The extent of damage done to human DNA in relation to time of exposure to artificial UV and high temperature above 100°C that make blood samples unsuitable for forensic analysis has not yet been determined, and using two methods of extraction the organic and kit methods. The gel electrophoresis was used to appear the bands.
Keywords
Main Subjects
- Bond J. W., and Hammond C.,2008 “The Value of DNA Material Recovered from Crime Scenes”, Journal of Forensic Sciences (Wiley-Blackwell), 53, 797- 801,
- Mitchell D., Paniker L., Lin K., and Fernandez A., 2015“Interspecific variation in the repair of UV damaged DNA in the genus Xiphophorus as a factor in the decline of the Rio Grande Platyfish”, Photochemistry and Photobiology, 91, 486-492.
- Gršković B., Dario Z, Maja P, Maja Jelena P, Dragan P, and Gordan M,2013 “Effect of ultraviolet C radiation on biological samples”, Croatian Medical Journal, 54, 263-271.
- Georgieva M., Nikolova I., Bonchev G., Katerova Z., and Todorova D.,2015 “A comparative analysis of membrane intactness and genome integrity in pea, barley, and wheat in response to UVC irradiation”, Turkish Journal of Botany, 39, 1008-1013.
- Fuminari.U, Shinji.M, Yasunori.T, Yukihiko.H, Shuya Y, Mako.Y, Elena.E, and Yasunori.M, et al.,2014 “Comparison of UVB and UVC effects on the DNA damage-response protein 53BP1 in human pancreatic cancer”, Journal Of Cellular Biochemistry, 115, 1724-1728.
- Yagura T., Makita K., Yamamoto H., Menck C. F. M., and Schuch A. P.2011 “Biological Sensors for Solar Ultraviolet Radiation”, Sensors (14248220), 11, 4277-4294.
- Shah S., “Spectroscopic Analysis of Ultraviolet Lamps for Disinfection of Air in Hospitals”, Water, Air & Soil Pollution Focus, 9, 529-537, 2009.
- Nair-Shalliker V., Fenech M., Forder P. M., Clements M. S., and Armstrong B. K.,2012 “Sunlight and vitamin D affect DNA damage, cell division and cell death in human lymphocytes”, a cross-sectional study in South Australia, Mutagenesis, 27, 609-614.
- Viki. S, Christina. A, Renny. S, Sandy. S, George. B, and Zalfa A. Abdel-Malek.,2014 “Significance of the melanocortin 1 receptor in the DNA damage response of human melanocytes to ultraviolet radiation”, Pigment Cell & Melanoma Research, 27, 601-610.
10. Bright J. A., Taylor D., Curran J. M., and Buckleton J. S., 2013“Degradation of forensic DNA profiles”, Australian Journal of Forensic Sciences, 45, 445-449.
11. Peters J. P., and Maher L. J., 2010 “DNA curvature and flexibility in vitro and in vivo”, Q. Rev. Biophys., 43, 23−63.
12. Kumar N., Chauhan A., and Gupta R., 2019“Effect of Fire on DNA and its profiling in homicide cases”, Forensic Res Criminol Int J, 7, 90-94,
13. Sambrook J., and Russell D. W.,2001 “Molecular Cloning: A Laboratory Manual”, New York, Cold Spring Harbor Laboratory Press.
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