Document Type : Research Paper

Authors

1 Biochemistry Branch - College of Medicine - Anbar University

2 Department of Life Sciences - College of Science - University of Anbar

Abstract

This study include investigation the effects of high temperature on the
chemical structure of RBCs membranes in thirty workers of the state coperation
of glass industries in Ramadi - Iraq , thirty technicians and administrators for
interior control and thirty volunteers as external control .
RBCs membranes isolation and characterization of lipids and protiens
and checking them by using TLC and gel electrophoresis respectively .
From the results of this study we observed proteins of RBCs membrane
decreased significantly (P<0.01) , cholesterol and phospholipids increased
significantly (P<0.01) , (lipidsproteins) ratio increased significantly (P<0.01) .
RBCs membranes proteins electrophoresis on (polyacrylamide gel)
showed the disappearance of number of proteins bands and decreased the
density of the other bands , while the separation on the (TLC) showed that there
were clear differences and there was change in the classes of RBCs membrane
lipids.

Main Subjects

[1]. ثرید، کولد. (1982) الترکیب الدقیق للخلیة الحیوانیة. ترجمة انور یعقوب وجماعته، جامعة الموصل. العراق.
[2]. Powers, LW. (1989) Diagnostic hematology: Clinical & technical Principles. Mosby Company.  United Sate of America.
[3]. Clark, MJ & Switzer, RL. (1977) Experimental Biochemistry. Second edition. Freeman Company, United State of America.  .
[4]. Gold, JC. & phillps, Ms. (1993) Effects of membrane lipids, proteins, and Cytoskeletal proteins on the Kinetics of cholesterrol exchange between high-density lipoproteins and human red blood cells, ghosts and microvesicles. Biochem. Biophys. Acta. , 1111 (1) , 103 – 110 .
[5]. AL-Qysi, H.MA. (1984) physical properties of mammalian Cell membranes and alteration after hyperthermia. Ph. D. thesis. University of waterloo. Canada.
[6]. Ando, M. Asanuma , Sh. Ushuda , M. Sasaki , K. et al. (1997) Age related effectes of heat stress on protective enzymes for peroxidase and micrlosomal monooxygenase in rat liver. J.Enviro. health. Perspecteives. , 105 (7), 726-733.
[7]. Wester, PO. (1989) Metabolism of minerals and trace elements in human disease: Magnesium and Cardiovascular disease. First edintion. Smith-Gordon. London.
[8]. Al-Gailani, BT. (1989) Deformability of human Red blood cell Ghosts. PH D thesis. University of Leeds. Canada.
[9]. Ghwarsh, KM. Abdel – Gayoum, AA. & AL-Alem, MG (1998) Lipid peroxidation in the erythrocytes of obese diabetic Subjects. Saudi. Medical Journal. , 19 (4); 453 – 458.
[10]. Zaltis, A. Zak, B, & Boyle, AJ. (1953). A new method for the direct determination of Serum cholesterol. J. lab. Clin. Med., 41, 486 – 492.
[11]. Lowry, OH. Rosebrough, NJ. Farr, AL. & Randall, RJ (1951) protein measurment with the folinphenol reagent. J. Biol. Chem., 193, 265– 275.
[12]. Hames, BD & Rickwood, D. (1984) Gel electrophoresis of proteins, Apractical approach. 3 rd edition, IRL press limited. England.
[13]. دونالد، سویر ولیم وجانیس هایفمان. (1989) تجارب کیمیائیة للطرق الآلیة ، ترجمة زهیر متی قصیر. جامعة بغداد –بیت الحکمة، مطبعة التعلیم العالی.