Author
Abstract
The bacterium staphylococcus aures was isolated from subcutaneous abcess by
using staphylococcal medium No-110 and diagnosed according to its cultural and micro
scopic characters , biochemical reactions and coagulase production . Sensitivity test of the
bacterium against antimicrobial agents was performed and the result appeared that the
bacterium was sensitive to cephlothin , ampicillin , ampiclox , tetracyclin , rifampicin ,
cloxamcillin ,fusidic acid , chloramphenicol and nitrofurontion , and resistant to cefotaxin ,
bacitracin neomycin, gentamycin , nalidixic aceid , penicllin , erythromycin and cloxacillin .
Curing experiment was accom plished using elevated growth temperature , to study
the role of plasmids in coding for the virulence factors , coagulase and hemolysin and
antibiotics resis tance , Result showed the partial loss of coogulase production through the
effect on RNA polymerase responsible of transcription of cluster structural genes with single
promoter and terminator produce coagulase and the method was not suitable for curing
hemolysin plasmid . Results of curing of plasmids responsible of antibiotics resistance
ensured the role of plasmids in resistance of the antibiotics penicillin , Erythromycin and
cloxacillin after physical loss of plasmid as proofed by gel electrophoresis .
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