Document Type : Research Paper

Authors

1 Department of Life Sciences/ College of Education/ University of Anbar

2 Department of Life Sciences/ College of Science/ University of Anbar

10.37652/juaps.2008.15349

Abstract

The study was conducted to produce the single cell protein by the use of Schanginia
aegyptiaca powder wastes of nutrient media and wheat hey. The aim was to grow local isolates
which were already diagnosed and sieved from (Aspergillus niger) in the liquid cultures
methods by using flasks in shaker incubators fermentation. The suitable conditions for
production such as components media, percentage of support materials, time of incubation and
type of best isolate for production were determined. Yield components of single cell protein
were checked. The yield, was then used in food and the breeding media of fruit fly (Drosophila
melanogaster). The results were as follows :-
1- Obtaining 10 local isolates inhabiting Schanginia aegyptiaca, three of them were selected
depending on the ratio of growth diameter which ranged between (5.60-6.40) cm on PDA
media.
2- The selected isolates Is-3, Is-4, Is-8 with A.niger-S showed an ability to grow on the
prepared media Schanginia aegyptiaca with a rate lower than the PDA media. The Is-8
isolate has been selected and diagnosed to belong to fungi A. niger-P8 with A.niger-S
isolate.
3- The liquid media, prepared from the powder of Schanginia aegyptiaca and wheat hey in 1:1
percentage produced the highest yield of single cell protein 9.11 g/Lit with A. niger-P8
isolate and 8.91 g/Lit with A. niger-S isolate during 8 days incubation period.
4- The highest percentage of the parent protein in biomass was 32.12 and 32.56 from A. niger-
P8 and A. niger-S isolates, respectively, and the produced protein contained low percentage
of nucleic acids RNA and DNA. The totals of their percentage were 5.18% and 5.32%, and
revealed that it contains 15 amino acids. The rates of amino acids were 81.4 and 80.5 g/100g
of protein for the two isolates A. niger-P8 and A. niger-S, respectively, and
characteristically the highest content of Aspartic; 16.12 and 15.62 g/100g of protein in the
liquid cultures for the A. niger-P8 and A. niger-S isolates, respectively. revealed that
production are free from toxins.
5- The use of microbial protein produced from the liquid fermentation for A. niger-P8 and
A. niger-S in feeding fruit fly Drosophila melanogaster instead of Corn starch in 100%
percentage gave the highest rate in the number of mature insect 1473 and 1381, for
the proteins of the two isolates A. niger-P8 and A. niger-S, respectively.

Keywords

Main Subjects

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