HISTOIMMUNOLOGICAL CHANGES IN THE URINARY SYSTEM OF WISTER ALBINO RAT RESULTED FROM Proteus mirabilis Infestations

Sh.Hamad, Kiffah; Saleh Shallal, Muhdi; Sh.Abid Al-Hayani, Hameed

doi:10.37652/juaps.2008.15295

Document Type : Research Paper

Authors

¹ College of Medicine / University of Anbar

² College of Education / University of Anbar

10.37652/juaps.2008.15295

Abstract

Urine samples of human's mid-stream urinary tract were collected
from patients (males and females)who suffered from signs of urinary tract
problems . These specified bacteria were isolated and classified according to
species .Bacteriological results indicated the existence of P.mirabilis, which was
the most familiar kind to cause these infections compared to other bacteria .
A dose of 9.0 × 109 alive P.mirabilis bacterial cells was injected after
being killed on bases of a limited period of time intraperitonially in each
laboratory rat ,signs of infections were noticed and later extra
histoimmunopathological changes were studied in accordance with their sickness
stages.
Results Showed the followings:
Bacterial infections were persistent and their severity was varied between
acute and chronic infections for all the period of the experiment .These
conditions were positive for all the urinary tract components, urethras ,urinary
bladder and urethra.
The strength of histoimmunopathological changes was varied among rats
of the same group for all the period of the experiment ,but in general these
changes varied from simple to moderate specially in the first week of inoculation
.However ,animals did have symptoms such as hyperemia and edema while
others suffered from necrosis , staffing of medulla , hyaline casts and
inflammatory edema .
Inflammation severity was increased after the first week and animals
suffered from a wide destruction in their kidney tissue because of inflammable
edema which experienced neutrophils penetration in the kidney tissues and the
collective tubules ; as a result, R.B.C `s were presented in their urine . Moreover,
in the second week their inflammatory conditions were variably extended to
chronic inflammation in the bladder through the urethras and then to urethra .
Also, swelling of the mucous membrane of the bladder and urethra. Rats may
have inflammable edema and chancre with severe edema in the inflamed cells

Keywords

Proteus mirabilis

Main Subjects

Biology

References

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2. Mostafa , M. H.; S.A. Sheweita and P.J.O .Connor . 2001: Urinary tract infectionً. Clinical microbiology reviews.J.P.97-111. Vol-12.No-5.

3. Zhao,H.;D.E .Johnson and H.L .Mobley.1999: Identification of Proteus and rpon - associated genes of uropathogenic Proteus mirabilis by negative selection in a moue model of ascending U.T.I . Microbiol: 154:185-95.

4. Meyrier,A. . 2000 Urinary Tract Infection: The textbook of medical microbiology (9)thed.Stough,London. P: 124-150.

5. Sobel,J2000.Pathogenesis of urinary tract infection.Infect Disً Clin .North Am.11(3):531-544.

6. Stamm,W.and P.Sternlieb.1993:Current concepts . Management of urinary tract infection in adult .New Engl. J .Med:329: (35): 1328-1334.

7. Maskell, R.; L. Pead and R.J.Hallett. 1975. Urinary pathogen in the male Br..J.Urol.47:691-694.

8. Jaffe,J.S.; W.E. Stamm and T.M. Hooton 1994. Collection of urine for culture. N. Engl. J. Med 331:617-618.

9. Liaw, S. J.; H. C. Lai; K. T. Luh and W.B.Wang.2000: Inhibition of virulence factor expression and swarming differentiation in Proteus mirabilis by nitrophenylglycerol. .J. Med. Microbial, P. 49: 725-731.

10. Brooks, G. F.; J. S. Butel and S.A. Morse. 2001. Jawetz, Melnick and Adelbergs medical microbiology (22)thed.Appleton and Lange, California..P.219-223.

11. Baron, E. J. and S.M .Finegold. 1990. Diagnostic microbiology, Bailey and Scotts. (8)thed Mosby.

12. Holt,J.G. ; N.R. Kreig; P.H.A.Sneath ; J.T.Staley and S.T.Williams.1994. Bergeys Manual of Determinative Bacteriology, (9)thed.Williams and Wilkins.

13. oneman,E.W.and R.G.Russell. 1997. Urinary calculi and urinary tract infection: a clinical and microbiological study..Nephrol.Suppl. 98:1-71.

14. Stamm,M.D. 2004:Urinary tract infection.New Engl.J.. Med. V.329- 335.

15. Buchaman,R .E. and Gibbous ,N. E.1994..: Bergeys Manual of Determi nation Bacteriology.(8)thed Williams and Wilkins Co.Baitimore.

16. Macfaddin,I .A. and S.Savage. 1985: Bacteraemia in diabetics. J. Inf.12:213-219.

17. Zunino,P.;C.Piccini and C.L.Fajardo.1999:Growth ,cellular differentation and virulence factor expression by Proteus mirabilis in vitro and in vivo.J. Med. Microbiol .48: 527- 534.Skin preparation before surgery lancet 2:1157-1160.

18. Bancroft, J. D. and A. Stevens, 1982.Theory and Practice of Histological techniques (2)nd ed churchill livingston .

19. Elaine, B.; A .Miriam and A. David.1985.Clinical evaluation of three rapid methods for the detection of significant Bacteria. Journal of clinical microbiology.J.P 62-67.V-22, No.1.

20. Tolson, D.L; B.A. Harrison; P. K. Latta; K. H. Lee and E. Altman. 1997: The expression of non-agglutinating fimbriae and its, role in Proteus mirabilis – adherence to epithelial cells.Can. J.Microb. 43: 709-17.

21. Linda, A. and C. Canine. 2003.: Pathogenic Bacteria, Bacteriology .Afr. J.Biomed.Res.Vol.5: 103-107.

22. Brooks,D. 1987.Concepts, Definitions and their practical value on over in view urinary tract infection. MTP Press.

23. Senior,B.W.and C.Hughes.1987:Production and properties of haemolysin from clinical isolates of the proteeae..J.Med.Micr obiol.24: 17-25.

24. Black,D.: 1998. Pyelonephritis in renal disease. (10)th ed. P:399.

25. Khaki,I. and A.S.AL-Kadi 2000:In vitro sensitivity of Pseudomonas aeru ginosa isolated from urinary tract infection .J. Med. Baghdad, 24:35-42.

26. Meallister,T.A. ; A.Pereival and J.G.Boyee, 1999. :Multicentric study of sensitivities of urinary tract pathogens.J. Med, 47:7-18.

27. Arean,V.M.,: 2000.Studies in the pathogensis of leptospirosis.Clinico pathologic evaluation of hepatic and renal function in experimental leptosprial infection.. Lab, 11:273-288.

28. Dove,G.A. ; A.J.Bailey, and H.E.Dewardner.2000. Diagnosis of urinary tract infection in general practice. Lab,2: 1281-3.

29. Brooks,D.and A.Maudar.1999.:Pathogensis of urethral syndrome in women and its diagnosis in general practice. P,2: 893-8.

30. Meers,P.D. 1999:The bacterological examination ofurine.J..72:229-344.

31. Guze,L.B. and P.B.Besson, 1999. Effect of ureteral ligation on the course of bacterial infection in the kidney of the rat. J. Med, 104:803-815.

32. Fierer,J.;L.Talner,and A.I.Braude:2000.Bacteremia in the pathogensis of retrograde E.coli pyelonephritis in the rat. J. Path,64: 443- 454.

33. ohnson, D.E.; R.G.Russell; C.V.Lockatell; J. C.Zuliy ;J.W.Warren and H.L.T.Mobley.1993.:Contribution of Proteus mirabilis urea’s to persistence. Urolithiasis and acute pyelonephritis in a mouse model of ascending urinary tract infection. Infect.Immun, 61:2748-2754.

34. Kalmanson, G.M. and L.B. Guze. 1999: pyelonephritis experimental ascen ding infection with progression of lesion in the absence of bacteria. P, 80:509-519.

35. Cotran, R.S.; l.O. Thrupp,; S.N. Hajjand .H.Kass.2000. Retrograde E.coli Pyelonephritis in rat a baterologic pathologic and fluorescent antibody study.J. Med, 61:987-1004.

36. Maclaren, D. M.1986: The siginficance of urease in Proteus pyelonephritis Abacterolgical study. J. Bac. 96:45-56.

37. Braude, A.I.;A.P.Shapiro,and J.Siemienski, 2001. Hematogenus pyelone phritis in rat .Relationship of bacterial species to the path ogensis of acut pyelonephritis. J. Bact... 77:270-280.

38. Glauser,M.P.; P.B.Franeioil, J.Bille and P.Meylan:1997.Effect of indcmethacin on the incidence of expermintal P.mirabilis pyelo nephritis. P, 40:529-533.

39. Shimamura, T. 1996:Mechanisms of renal tissue destruction in an experim ental acut pyelonephritis..P34:34-42.

40. Breslau, N.A and K.Sakhacc, 1994: Pathophysiology of non-hyper caleiuric causes of stons in renal stone disease. P:47.

41. Shapiro,A.P. and A.I.Braude1998.:Hematogenus pyelonephritis in rat rela tionship of bacterial species to the pathogenesis of chronic.

42. More, I.A.R. 1995:Kidney and urinary tract in Muirs textbook of pathology. (15)th ed..P,22.

43. Thomas,C.and Tang,J.D.1994: Experimental pyelonephritis and papillary necrosis in the Gunnrat.. Pathology, 17:420-428.

HISTOIMMUNOLOGICAL CHANGES IN THE URINARY SYSTEM OF WISTER ALBINO RAT RESULTED FROM Proteus mirabilis Infestations

References

References

1. Zhao, H.; R.B. Thompson; V. Locketell and H. L. Mobley.1998: Use of green fluorescent protein to assess urease gene expression by uro-pathogenic Proteus mirabilis during experimental ascending U.T.I. infect. and Immune.. 66: 330-5.

2. Mostafa , M. H.; S.A. Sheweita and P.J.O .Connor . 2001: Urinary tract infectionً. Clinical microbiology reviews.J.P.97-111. Vol-12.No-5.

3. Zhao,H.;D.E .Johnson and H.L .Mobley.1999: Identification of Proteus and rpon - associated genes of uropathogenic Proteus mirabilis by negative selection in a moue model of ascending U.T.I . Microbiol: 154:185-95.

4. Meyrier,A. . 2000 Urinary Tract Infection: The textbook of medical microbiology (9)thed.Stough,London. P: 124-150.

5. Sobel,J2000.Pathogenesis of urinary tract infection.Infect Disً Clin .North Am.11(3):531-544.

6. Stamm,W.and P.Sternlieb.1993:Current concepts . Management of urinary tract infection in adult .New Engl. J .Med:329: (35): 1328-1334.

7. Maskell, R.; L. Pead and R.J.Hallett. 1975. Urinary pathogen in the male Br..J.Urol.47:691-694.

8. Jaffe,J.S.; W.E. Stamm and T.M. Hooton 1994. Collection of urine for culture. N. Engl. J. Med 331:617-618.

9. Liaw, S. J.; H. C. Lai; K. T. Luh and W.B.Wang.2000: Inhibition of virulence factor expression and swarming differentiation in Proteus mirabilis by nitrophenylglycerol. .J. Med. Microbial, P. 49: 725-731.

10. Brooks, G. F.; J. S. Butel and S.A. Morse. 2001. Jawetz, Melnick and Adelbergs medical microbiology (22)thed.Appleton and Lange, California..P.219-223.

11. Baron, E. J. and S.M .Finegold. 1990. Diagnostic microbiology, Bailey and Scotts. (8)thed Mosby.

12. Holt,J.G. ; N.R. Kreig; P.H.A.Sneath ; J.T.Staley and S.T.Williams.1994. Bergeys Manual of Determinative Bacteriology, (9)thed.Williams and Wilkins.

13. oneman,E.W.and R.G.Russell. 1997. Urinary calculi and urinary tract infection: a clinical and microbiological study..Nephrol.Suppl. 98:1-71.

14. Stamm,M.D. 2004:Urinary tract infection.New Engl.J.. Med. V.329- 335.

15. Buchaman,R .E. and Gibbous ,N. E.1994..: Bergeys Manual of Determi nation Bacteriology.(8)thed Williams and Wilkins Co.Baitimore.

16. Macfaddin,I .A. and S.Savage. 1985: Bacteraemia in diabetics. J. Inf.12:213-219.

17. Zunino,P.;C.Piccini and C.L.Fajardo.1999:Growth ,cellular differentation and virulence factor expression by Proteus mirabilis in vitro and in vivo.J. Med. Microbiol .48: 527- 534.Skin preparation before surgery lancet 2:1157-1160.

18. Bancroft, J. D. and A. Stevens, 1982.Theory and Practice of Histological techniques (2)nd ed churchill livingston .

19. Elaine, B.; A .Miriam and A. David.1985.Clinical evaluation of three rapid methods for the detection of significant Bacteria. Journal of clinical microbiology.J.P 62-67.V-22, No.1.

20. Tolson, D.L; B.A. Harrison; P. K. Latta; K. H. Lee and E. Altman. 1997: The expression of non-agglutinating fimbriae and its, role in Proteus mirabilis – adherence to epithelial cells.Can. J.Microb. 43: 709-17.

21. Linda, A. and C. Canine. 2003.: Pathogenic Bacteria, Bacteriology .Afr. J.Biomed.Res.Vol.5: 103-107.

22. Brooks,D. 1987.Concepts, Definitions and their practical value on over in view urinary tract infection. MTP Press.

23. Senior,B.W.and C.Hughes.1987:Production and properties of haemolysin from clinical isolates of the proteeae..J.Med.Micr obiol.24: 17-25.

24. Black,D.: 1998. Pyelonephritis in renal disease. (10)th ed. P:399.

25. Khaki,I. and A.S.AL-Kadi 2000:In vitro sensitivity of Pseudomonas aeru ginosa isolated from urinary tract infection .J. Med. Baghdad, 24:35-42.

26. Meallister,T.A. ; A.Pereival and J.G.Boyee, 1999. :Multicentric study of sensitivities of urinary tract pathogens.J. Med, 47:7-18.

27. Arean,V.M.,: 2000.Studies in the pathogensis of leptospirosis.Clinico pathologic evaluation of hepatic and renal function in experimental leptosprial infection.. Lab, 11:273-288.

28. Dove,G.A. ; A.J.Bailey, and H.E.Dewardner.2000. Diagnosis of urinary tract infection in general practice. Lab,2: 1281-3.

29. Brooks,D.and A.Maudar.1999.:Pathogensis of urethral syndrome in women and its diagnosis in general practice. P,2: 893-8.

30. Meers,P.D. 1999:The bacterological examination ofurine.J..72:229-344.

31. Guze,L.B. and P.B.Besson, 1999. Effect of ureteral ligation on the course of bacterial infection in the kidney of the rat. J. Med, 104:803-815.

32. Fierer,J.;L.Talner,and A.I.Braude:2000.Bacteremia in the pathogensis of retrograde E.coli pyelonephritis in the rat. J. Path,64: 443- 454.

33. ohnson, D.E.; R.G.Russell; C.V.Lockatell; J. C.Zuliy ;J.W.Warren and H.L.T.Mobley.1993.:Contribution of Proteus mirabilis urea’s to persistence. Urolithiasis and acute pyelonephritis in a mouse model of ascending urinary tract infection. Infect.Immun, 61:2748-2754.

34. Kalmanson, G.M. and L.B. Guze. 1999: pyelonephritis experimental ascen ding infection with progression of lesion in the absence of bacteria. P, 80:509-519.

35. Cotran, R.S.; l.O. Thrupp,; S.N. Hajjand .H.Kass.2000. Retrograde E.coli Pyelonephritis in rat a baterologic pathologic and fluorescent antibody study.J. Med, 61:987-1004.

36. Maclaren, D. M.1986: The siginficance of urease in Proteus pyelonephritis Abacterolgical study. J. Bac. 96:45-56.

37. Braude, A.I.;A.P.Shapiro,and J.Siemienski, 2001. Hematogenus pyelone phritis in rat .Relationship of bacterial species to the path ogensis of acut pyelonephritis. J. Bact... 77:270-280.

38. Glauser,M.P.; P.B.Franeioil, J.Bille and P.Meylan:1997.Effect of indcmethacin on the incidence of expermintal P.mirabilis pyelo nephritis. P, 40:529-533.

39. Shimamura, T. 1996:Mechanisms of renal tissue destruction in an experim ental acut pyelonephritis..P34:34-42.

40. Breslau, N.A and K.Sakhacc, 1994: Pathophysiology of non-hyper caleiuric causes of stons in renal stone disease. P:47.

41. Shapiro,A.P. and A.I.Braude1998.:Hematogenus pyelonephritis in rat rela tionship of bacterial species to the pathogenesis of chronic.

42. More, I.A.R. 1995:Kidney and urinary tract in Muirs textbook of pathology. (15)th ed..P,22.

43. Thomas,C.and Tang,J.D.1994: Experimental pyelonephritis and papillary necrosis in the Gunnrat.. Pathology, 17:420-428.

Volume 2, Issue 2August 2008Page 36-51

Volume 2, Issue 2
August 2008
Page 36-51