Document Type : Research Paper

Authors

1 University of Anbar - College of Education and Pure Sciences

2 University of Anbar - Center for Desert Studies

10.37652/juaps.2015.124510

Abstract

The current study Included (100) women suffering from recurrent infection of the urinary tract revisions for hospitals and civil laboratories in Anbar province, the category of age among women (10-50 years), in addition to (40) women do not have any history of infection in the same age group as a control sample for comparison. The study achieved in period between 03/09/2013 to 27/12/2014 .The highest recorded rate of infection in the age ranged between (21-35 years) (48%), also the high incidence observed were among married women (62%), and most infection were in women of villages and rural areas(67%). The current study involved measuring the level of concentration of Tamm horsfall protein (THP) the study showed a significant decrease at P≤0.05 in (43%) and the rate of individuals (6.2 ± 38.3) Ng / ml compared with control sample, which was (7.4 ± 256.2) Ng / ml. The study also included molecular tests (for women suffering from UTI and THP concentration disorders) using Polymerase Chain Reaction technique and using three specific primers for the detection of any trouble in Uromodulin gene and its relationship to UTI. The results showed a significant increase P≤0.05 for the study sample represents (24,19,18) absence time of primers bands in some cases to appear different bands from the expected size compared with the control sample that did not show any of the previous cases.

Keywords

Main Subjects

1-Addose ,S.A.(2000) . Urinary tract infection among burn patients with special reference to Psendomonas aeruginosa.kufa , Med . J. , 3(1) : 55-61 .
2-Moges, A.F.; Genetu, A. and Mengistu, G. (2002) “Antibiotic sensitivities of common bacterial pathogens in urinary tract infections” East Afric. Medi. J. Vol.79.No. 3, PP. 167-169
3-O'Dell, K. (2011). Pharmacologic Management of AsymptomaticBacteriuria and Urinary TractInfections in Women. Journal ofMidwifery &Women’s Health 56.(3):248-
4-Nicolle,L.E. (2010): Asymptomatic bacteriuria Urogenital Infections.chapter 5,European Association of Urology.pp:299-314.
5-Luca,  R .; Francesco, S .; Atonio,  A  .; Gianmacro,  G. and  Olivier,  D. (2011) . The rediscovery of uromodulin (Tamm-Horsfall Protein) :from tubule-interstitial nephronpathway to chronic Kidny disease , university of zurich
6-Schaeffer, C.; Santambrogio, S.; Perucca, S.; Casari, G ,and Rampoldi, L(2009).Analysis of uromodulin polymerization provides new insights into the mechanisms regulating ZP domain-mediated protein assembly. Mol Biol Cell. Jan;20(2):589-99.
7-Pak, J.; Pu, Y.; Zhang, ZT.;Hasty, DL. And Wu, XR. (2001):Tamm-Horsfall protein binds to type1 fimbriated Escherichia coli and prevents E. coli from binding to uroplakin Iaand Ib receptors. J Biol Chem. Mar 30;276(13):9924-30.
8-Rampoldi, L.; Caridi, G.; Santon, D.; Boaretto, F.; Bernascone, I.; Lamorte, G.; Tardanico, R.; Dagnino, M.; Colussi, .; Scolari, F.; Ghiggeri, GM.; Amoroso, A. and Casari, G.(2003) Allelism of MCKD, FJHN and GCKD caused by impairment of uromodulin export dynamics. Hum Mol Genet. Dec 15;12(24):3369-84
-9الحسینی ,رعد خلیل (1996).تشخیص وتنمیط بکتریا التهابات الجهاز البولی وقابلیتها على انتاج الهیمولایسین ومقاومة المضادات الحیویة ،أطروحة دکتوراه ,کلیة العلوم ,قسم علوم الحیاة ، الجامعة المستنصریة ,بغداد, العراق
-10خورشید ، ثری احمد (2005).دراسة جرثومیة لبعض مسببات اخماج المسلک البولی للمرضى فی مستشفى ازادی العام فی مدینة کرکوک.اطروحه ماجستیر ،کلیة التربیة ،جامعة تکریت.
-11عوض ،هالة عبد الخالق(2005).التأثیر التثبیطی لبعض المستخلصات النباتیة على البکتریاالمسببة لخمج المجاری البولیة. اطروحه ماجستیر، کلیة التربیة للبنات ,جامعة تکریت,العراق.
-12نایف، حمدی عبد الله(2007).ظهور المکورات العنقودیة الرمیةStaphylococcussaprophyticusفی خمج المجاری البولیة مع الترکیز على عوامل ضراوتها.اطروحة دکتواره ،کلیة العلوم ،جامعة الأنبار ،العراق
-13عبد الحمزه, عباس مهدی (2010).دراسة العوامل المتعلقة بالإصابة بمرض إلتهاب المجاری البولیة فی ناحیة الامامی بمحافظة بابل,کلیة التربیة الأساسیة, جامعة بابل,العدد(2) :318-327
14-Develay, A.F.; Fontaine, A.; Guiot, M.; Lecourt, A.; Rodriguez, M. and Brodin, M. (1996). Analysis of pediatric home care services of the assistance. Arch. Pediatr., 3(1): 28-34.
15-Fistershammer,P K.;Christoph, K.; Leitner, F.; Stockl, J.; Majdic, O.;Weichhart,T.;Sobanov,Y.;Bochkov,V.; Saemann, M.;Zlabinger,G.andSteinberger,P.(2008):Identification of the scavenger receptorsSREC-1, Cla-1 (SR-B1) and SR-A1 as cellular receptor for Tamm-Horsfall protein. J. Leukoc.Biol.(83)131-138.
16-Kobayashi,K . and  Fukuoka , S . (2001). Condition for solubilization of Tamm-Horsfall protiens/uromodulinn in human  urine and  establishment  of  a  sensitive  and ocurate  enzyme-linked immunosorbent assay  (ELIZA)method Arch BiochemoBiophys.388 :113-120
17-Devuyst, O.; Dahan, K. and Pirson, Y.(2005). Tamm-Horsfall protein or uromodulin: new ideas about an old molecule. Nephrol Dial Transplant. Jul;20(7):1290-4
18-Wai-Hoe Lau, Wing-Seng Leong, Zhari Ismail, Lay-Harn Gam.(2008): Qualification and application of an ELISA for the determination of Tamm Horsfall Protein (THP) in human and its use for screening of Kidney Stone Disease.Int J BiolSci, 4(4):215-222.(Iraq virtual science library).
19-Carvalho ,M.; Mulinari ,M.A and Nakagawa ,Y (2002).Role of Tamm-Horsfall protein and uromodulin in calcium oxalate crystallization,Brazilian Journal of Medical and Biological Research (35): 1165-1172
20-Hess, B.; Jaggi, M.; Zipperle, L. and Jaeger, P.(1995). Reduced carbohydrate content ofTamm-Horsfall glycoprotein (THP) fromsevere recurrent renal calcium stoneformers (RCSF). Journal of the AmericanSociety of Nephrology, 6: 949
21-Bleyer, AJ.; Hart, TC.; Shihabi, Z.; Robins, V.and Hoyer, JR.(2004) Mutations in the uromodulin gene decrease urinary excretion of Tamm-Horsfall protein. Kidney Int. Sep; 66(3):974-7.
22-Dahan, K.; Devuyst, O.; Smaers, M.; Vertommen, D.; Loute, G.;Poux, J.M.; Viron, B.; Jacquot, C.; Gagnadoux, M.F.; Chauveau, D. et al. (2003) A cluster of mutations in the UMOD gene causes familial juvenile hyperuricemic nephropathy with abnormal expression of uromodulin. J. Am. Soc. Nephrol.,(14): 2883–2893.
23-Scott, SP.; Bendix, R.; Chen, p. et al (2002).Missense Mutation but not allelic variants alter the function of ATMby dominant interference in patient with brest cancer.Proc Natl Acad Sci USA(99):925-930.